Basic Laboratory Procedures in Clinical Bacteriology: Part I. Bacteriological investigations: Blood


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Basic Laboratory Procedures in Clinical Bacteriology (WHO; 1991; 128 pages)
	Preface
	Introduction
	Quality assurance in microbiology
		Introduction
		Definitions
		Internal quality control
		External quality assessment
	Part I. Bacteriological investigations
		Blood
			Introduction
			Causes of bacteraemia
			Blood collection
			Blood-culture media
			Processing of blood cultures
		Cerebrospinal fluid
			Introduction
			Collection and transportation of specimens
			Macroscopic inspection
			Microscopic examination
			Preliminary identification
			Susceptibility testing
		Urine
			Introduction
			Specimen collection
			Culture and interpretation
			Interpretation of quantitative urine culture results
			Identification
			Susceptibility tests
		Stool
			Introduction
			Collection of faecal specimens
			Collection of rectal swabs
			Examination of specimens
			Preparation of faecal suspension
			Inoculation of agar plates
		Lower respiratory tract infections
			Introduction
			The most common infections
			Collection of sputum specimens
			Processing of sputum in the laboratory (for non-tuberculous infections)
			Culture for Mycobacterium tuberculosis
			General note on safety
		Upper respiratory tract infections
			Introduction
			The normal flora of the pharynx
			Bacterial agents of pharyngitis
			Collection and dispatch of specimens
			Direct microscopy
			Culture and identification
			Susceptibility testing
		Sexually transmitted diseases
			Introduction
			Urethritis in men
			Genital specimens from women
			Specimens from genital ulcers
		Purulent exudates, wounds, and abscesses
			Introduction
			Commonly encountered clinical conditions and the most frequent etiological agents
			Collection and transportation of specimens
			Macroscopic evaluation
			Microscopic examination
			Culture
			Identification
			Susceptibility testing
		Anaerobic bacteriology
			Introduction
			Description of bacteria in relation to oxygen requirement
			Bacteriology
		Antimicrobial susceptibility testing
			Introduction
			General principles of antimicrobial susceptibility testing
			Clinical definition of terms “resistant” and “susceptible”: the three-category system
			Indications for routine susceptibility tests
			Choice of drugs for routine susceptibility tests in the clinical laboratory
			The modified Kirby-Bauer method
			Direct versus indirect susceptibility tests
			Technical factors influencing the size of the zone in the disc diffusion method
			Quality control
	Part II. Essential media and reagents for isolation and identification of clinical pathogens
		Introduction
		Priority grading of pathogens, culture media, and diagnostic reagents
			Blood culture
			Cerebrospinal fluid specimens
			Urine
			Stool culture
			Lower respiratory tract
			Upper respiratory tract
			Urogenital specimens for agents of sexually transmitted diseases
			Pus and exudates
			List of recommended culture media and diagnostic reagents for the intermediate microbiological laboratory
	Selected further reading
	Selected WHO publications of related interest
	Back Cover

Blood

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